Title: Bacterial Polyglucuronic Acid/Alginate/Carbon Nanofibers Hydrogel Nanocomposite as a Potential Scaffold for Bone Tissue Engineering
Journal: Materials
Author: 1. Zahra Ebrahimvand Dibazar, 2. Mahnaz Mohammadpour, 3. Hadi Samadian, 4. Soheila Zare, 5. Mehdi Azizi, 6,7. Masoud Hamidi , 8. Redouan Elboutachfaiti, Emmanuel Petit, 9,10. Cédric Delattre
Year: 2022
Address: 1. Department of Oral and Maxillo Facial Medicine, Faculty of Dentistry, Tabriz Asad University of Medical Sciences, Tabriz 5166616471, Iran 2. Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran 1411713116, Iran 3. Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah 6715847141, Iran 4. Student Research Committee, Zanjan University of Medical Sciences, Zanjan 7797845157, Iran 5. Department of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan 6517838636, Iran 6. BioMatter-Biomass Transformation Lab (BTL), École Polytechnique de Bruxelles, Université Libre de Bruxelles, Avenue F.D. Roosevelt, 50-CP 165/61, 1050 Brussels, Belgium 7. Department of Medical Biotechnology, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht 4188794755, Iran 8. UMRT INRAE 1158 BioEcoAgro, Laboratoire BIOPI, Université de Picardie Jules Verne, IUT d’Amiens, 80025 Amiens, France 9. Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, 63000 Clermont-Ferrand, France 10. Institut Universitaire de France (IUF), 1 Rue Descartes, 75005 Paris, France
Abstract: 3D nanocomposite scaffolds have attracted significant attention in bone tissue engineering applications. In the current study, we fabricated a 3D nanocomposite scaffold based on a bacterial polyglucuronic acid (PGU) and sodium alginate (Alg) composite with carbon nanofibers (CNFs) as the bone tissue engineering scaffold. The CNFs were obtained from electrospun polyacrylonitrile nanofibers through heat treatment. The fabricated CNFs were incorporated into a PGU/Alg polymeric solution, which was physically cross-linked using CaCl2 solution. The fabricated nanocomposites were characterized to evaluate the internal structure, porosity, swelling kinetics, hemocompatibility, and cytocompatibility. The characterizations indicated that the nanocomposites have a porous structure with interconnected pores architecture, proper water absorption, and retention characteristics. The in vitro studies revealed that the nanocomposites were hemocompatible with negligible hemolysis induction. The cell viability assessment showed that the nanocomposites were biocompatible and supported bone cell growth. These results indicated that the fabricated bacterial PGU/Alg/CNFs hydrogel nanocomposite exhibited appropriate properties and can be considered a new biomaterial for bone tissue engineering scaffolds.
Keywords: polyglucuronic acid; Sinorhizobium meliloti M5N1CS; carbon nanofibers; hydrogel; bone tissue engineering; nanocomposites
Application: Tissue Engineering, Scaffold
Product Model 1: Electrospinning Setup
Product Model 2:
URL: #https://www.mdpi.com/1996-1944/15/7/2494#