Title: In vitro evaluation of Zeolite-nHA blended PCL/PLA nanofibers for dental tissue engineering
Journal: Materials Chemistry and Physics
Author: 1. Soodabeh Davaran, 1,2. Sanam Mohandesnezhad, 1,8. Mohammad Samiei, 2,5,6. Arash Goodarzi, 3. Younes Pilehvar-Soltanahmadi, Nosratollah Zarghami, 4. Effat Alizadeh, 7. Masoumeh Khatamian, 8. Marzieh Aghazadeh, 9. Abolfazl Akbarzadeh
Year: 2020
Address: 1. Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 3. Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran 4. Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran 5. Department of Tissue Engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran 6. Nancommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran 7. Department of Inorganic Chemistry, University of Tabriz, 51666-14779, Tabriz, Iran 8. Department of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran 9. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Abstract: This study aimed to investigate the potential of a composite poly(lactic acid)/polycaprolactone (PLA/PCL) blend electrospun nanofiber containing both nano-hydroxyapatite (nHA) and zeolite for promoting the proliferation of human dental pulp-derived stem cells (hDPSCs) with possible application in dental tissue engineering. For this purpose, nHA and Zeolite were synthesized via the hydrothermal method and their morphological features and crystals properties were studied by FESEM and X-ray diffraction, respectively. PCL-PLA/nHA/zeolite nanofibers were fabricated via electrospinning and characterized through FT-IR and water contact angle measurement methods. Then, the viability and adhesion of hDPSCs on the fibers were investigated by MTT assay and FESEM, respectively. The results showed that the viability of hDPSCs on the PCL-PLA/Zeolite scaffolds was significantly improved after 1, 7 and 14 days of culturing and maximum enhancing on the cell viability was detected after 3 days on PCL-PLA/nHA scaffolds (P≤0.001). After 7 and 14 days of incubation, cell growth on the scaffolds containing both nHA and Zeolite was better than the nanofibers which were loaded with nHA alone. Briefly, these results revealed that Zeolite-loaded nanofiber is the most suitable scaffold for bone and tooth tissue engineering applications. More studies are required to investigate the efficiency of zeolite-based nanofibrous scaffolds for the development of artificial scaffolds for dental tissue regeneration.
Keywords: Dental Pulp Stem Cells, Nanofibers, Zeolite, nHA, PLA, PCL
Application: Tissue Engineering
Product Model 1: Electroris
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/abs/pii/S025405842030523X#