Title: Differentiation of mesenchymal stem cells into neuron-like cells using composite 3D scaffold combined with valproic acid induction
Journal: Journal of Biomaterials Applications
Author: 1. Sadegh Ghorbani, Taki Tiraihi, 2. Masoud Soleimani
Year: 2018
Address: 1. Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran 2. Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Islamic Republic of Iran
Abstract: The nervous system has little capacity for self-repair after injury because neurons cannot proliferate owing to lack of suitable microenvironment. Therefore, neural tissue engineering that combines neural stem, scaffolds, and growth factors may improve the chance of restoration of damaged neural tissues. A favorable niche for neural regeneration would be both fibrous and electrically conductive scaffolds. Human Wharton jelly-derived mesenchymal stem cells were seeded on wetelectrospun 3D scaffolds composed of poly lactic acid coated with natural polymers including alginate and gelatin, followed by a multi-wall carbon nanotube coating. The results show that a wet-electrospun poly lactic acid scaffold at a concentration of 15% w/v had higher porosity (above 80%) than other concentrations. Moreover, the coated scaffold supported the growth of human Wharton jelly-derived mesenchymal stem cells in 3D culture, and were incubated for 21 days with 1 mM valproic acid as the inducer resulted in improvement in human Wharton jelly-derived mesenchymal stem cells differentiation into neuronlike cells immunoreactivity to nestin, Map2, and neuron specific enolase (NSE), which were also consistent with reverse transcription polymerase chain reaction (RT-PCR) and quantitive Reverse transcription polymerase chain reaction (qRT-PCR) results. The conclusion is that the 3D composite nanofiber poly lactic acid scaffold improved the transdifferentiation of human Wharton jelly-derived mesenchymal stem cells into neuron-like cells.
Keywords: Scaffold, mesenchymal stem cells, neurons, regeneration, valproic acid
Application: Scaffold, Tissue Engineering
Product Model 1: High Voltage Power Supply
Product Model 2:
URL: #https://journals.sagepub.com/doi/abs/10.1177/0885328217741903#