Title: The Differentiation of Human Endometrial Stem Cells into Neuron-Like Cells on Electrospun Pan-Derived Carbon Nanofibers with Random and Aligned Topographies
Journal: Molecular neurobiology
Author: 1. Hossein Ghanbari, Reza Faridi-Majidi, 1,2. Esmaeil Mirzaei, 3. Jafar Ai, Somayeh Ebrahimi-Barough, 4. Javad Verdi
Year: 2015
Address: 1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran 3. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 4. Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract: Electrospun carbon nanofibers (CNFs) have great potential for applications in neural tissue regeneration due to their electrical conductivity, biocompatibility, and morphological similarity to natural extracellular matrix. In this study, we cultured human endometrial stem cells (hEnSCs) on electrospun CNFs with random and aligned topographies and demonstrated that hEnSCs could attach, proliferate, and differentiate into neural cells on both random and aligned CNFs. However, the proliferation, differentiation, and morphology of cells were affected by CNF morphology. Under the proliferative condition, hEnSCs showed lower proliferation on aligned CNFs than on random CNFs and on tissue culture plate (TCP) control. When cultured on aligned CNFs in neural induction media, hEnSCs showed significant upregulation of neuronal markers, NF-H and Tuj-1, and downregulation of neural progenitor marker (nestin) compared to that on random CNFs and on TCP. In contrast, hEnSCs showed higher expression of nestin and slight upregulation of oligodendrocyte marker (OLIG-2) on random CNFs compared to that on aligned CNFs and on TCP. SEM imaging revealed that differentiated cells extended along the CNF main axis on aligned CNFs but stretched multidirectionally on random CNFs. These findings suggest electrospun CNFs as proper substrate for stem cell differentiation into specific neural cells.
Keywords: Electrospun carbon nanofibers . Surface topography . Human endometrial stem cell . Differentiation . Neuron-like cells
Application: Tissue Engineering
Product Model 1: Electroris
Product Model 2:
URL: https://link.springer.com/article/10.1007/s12035-015-9410-0#="https://link.springer.com" & "/article/10.1007/s12035-015-9410-0"#