Title: Immobilization of bromelain and ZnO nanoparticles on silk fibroin nanofibers as an antibacterial and anti-inflammatory burn dressing
Journal: International Journal of Pharmaceutics
Author: 1. Maede Hasannasab, Jhamak Nourmohammadi, AzadehGhaee, 2,3. Mohammad Mehdi Dehghan
Year: 2021
Address: 1. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran 2. Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 3. Institute of Biomedical Research, University of Tehran, Tehran, Iran
Abstract: Burns is a critical fatal event due to the risk of infection and complex inflammatory cascades. This study aimed to fabricate and characterize a new antibacterial and anti-inflammatory dressing for second-degree burns by the immobilization of bromelain and zinc oxide nanoparticles on silk fibroin nanofibers. Thus, electrospun silk nanofibers with an average fiber diameter of 345 nm were prepared and then grafted with acrylic acid after exposure to O2 plasma. Next, bromelain was immobilized on the modified SF nanofibers (SF-Br). Subsequently, different amounts of ZnO NPs coated with polydopamine were immobilized on the SF-Br nanofibers. The successful immobilization of bromelain and ZnO NPs on the SF nanofibers was proved by SEM, EDS, and FTIR analysis. The loading efficiency of bromelain was 85.63%, and activity ranged between 88% and 92%. The crystallinity of SF nanofibers decreased after the addition of bromelain and ZnO NPs, which increased the bromelain and zinc ions released from the dressing. Antibacterial activity has improved with the addition of ZnO NPs. The amounts of bromelain released from the dressings are not toxic to fibroblasts. Moreover, fibroblast attachment and proliferation enhanced at lower ZnO amounts, while there was an inverse trend at high doses of ZnO NPs. In vivo studies showed that treating the burn with silk fibroin-bromelain-ZnO NPs enhanced the healing process and considerably lowered the inflammatory response at the wound. Overall, the dressing presented here offers excellent potential for burn management.
Keywords: Immobilization, Nanofiber, ZnO nanoparticle, Anti-inflammatory, Antibacterial
Application: Antibacterial Properties, Wound Dressing
Product Model 1: Electroris
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/abs/pii/S0378517321010334#