Title: Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa
Journal: BMC biotechnology
Author: 1. Khosro Zamani, Rezvan Golmoradi, Shabnam Razavi, Gholam‑Reza Irajian, 2. Noushin Allah‑Bakhshi, Faezeh Akhavan, Mahdieh Yousefi, Mahyar Gerami, 3. Moazzameh Ramezani, 4. Horacio Bach, 5. Ali Pakdin‑Parizi, 6. Majid Tafrihi, 7. Fatemeh Ramezani
Year: 2021
Address: 1. (a) Microbial Biotechnology Research Center, Iran University of Medical Sciences,Tehran, Iran. (b) Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. 2. Department of Biology, Sana Institute of Higher education, Sari, Iran. 3. Department of Biology, Urmia University, Urmia, Iran. 4. Division of Infectious Diseases, Department of Medicine, University of British Columbia, Vancouver, BC, Canada 5. Sari Agricultural Sciences and Natural Resources University, Sari, Iran. 6. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran. 7. Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran.
Abstract: Background: Antibiotics have been widely used for the treatment of bacterial infections for decades. However, the rapid emergence of antibiotic-resistant bacteria has created many problems with a heavy burden for the medical community. Therefore, the use of nanoparticles as an alternative for antibacterial activity has been explored. In this context, metal nanoparticles have demonstrated broad-spectrum antimicrobial activity. This study investigated the antimicrobial activity of naked cerium oxide nanoparticles dispersed in aqueous solution (CNPs) and surface-stabilized using Pseudomonas aeruginosa as a bacterial model. Methods: Gelatin-polycaprolactone nanofibers containing CNPs (Scaffold@CNPs) were synthesized, and their effect on P. aeruginosa was investigated. The minimum inhibitory and bactericidal concentrations of the nanoparticls were determined in an ATCC reference strain and a clinical isolate strain. To determine whether the exposure to the nanocomposites might change the expression of antibiotic resistance, the expression of the genes shv, kpc, and imp was also investigated. Moreover, the cytotoxicity of the CNPs was assessed on fibroblast using flow cytometry. Results: Minimum bactericidal concentrations for the ATCC and the clinical isolate of 50 μg/mL and 200 μg/mL were measured, respectively, when the CNPs were used. In the case of the Scaffold@CNPs, the bactericidal effect was 50 μg/mL and 100 μg/mL for the ATCC and clinical isolate, respectively. Interestingly, the exposure to the Scaffold@ CNPs significantly decreased the expression of the genes shv, kpc, and imp. Conclusions: A concentration of CNPs and scaffold@CNPs higher than 50 μg/mL can be used to inhibit the growth of P. aeruginosa. The fact that the scaffold@CNPs significantly reduced the expression of resistance genes, it has the potential to be used for medical applications such as wound dressings.
Keywords: Cerium oxide nanoparticles, Nanofiber, Antibiotic resistance, Pseudomonas aeruginosa, Gene expression, Cytotoxicity, Clinical isolate
Application: Antibacterial Properties
Product Model 1: Electroris
Product Model 2:
URL: #https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-021-00727-1#