Title: Diltiazem-loaded electrospun nanofibers as a new wound dressing: fabrication, characterization and experimental wound healing
Journal: Pharmaceutical Development and Technology
Author: 1. Ramin Seyedian, Sasan Zaeri, 2. Elham Shabankareh Fard, 3. Seyede Sahar Hashemi, Hossein Hasanzadeh, 4. Majid Assadi
Year: 2021
Address: 1. Department of Pharmacology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran. 2. Department of Environmental Health Engineering, Faculty of Health, Bushehr University of Medical Sciences, Bushehr, Iran. 3. Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran. 4. Nuclear Medicine and Molecular Imaging Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.
Abstract: Calcium channel blockers such as diltiazem have recently been investigated for their wound healing potential. The aims of this study were to fabricate diltiazem-loaded nanofibers for a new wound dressing and investigate their beneficial properties for wound healing. Nanofibers were electrospun using polyvinyl alcohol solution containing 0%, 2% or 4% diltiazem. Fibers were characterized in terms of physicochemical properties, drug release and fibroblast viability, and in animal wound healing assays. Compared to other formulations, nanofibers containing 4% diltiazem showed thin fiber size (152.7 nm), high porosity (88.4%), high swelling (110.4%), low water contact angle (29.1°) and little weight loss (17.3%). Drug release from 4%-diltiazem nanofibers showed good fit to a Korsmeyer-Peppas model, suggesting a non-Fickian release mechanism (R2 = 96%, n = 0.52). In vitro, 4%-diltiazem mats were not cytotoxic and enhanced fibroblast proliferation by 263% after 5 days of treatment compared to control mats. In vivo, wounds treated with this mat for 14 days showed the smallest size (14.7%) and better histopathologic characteristics compared to other wounds. The 4%-diltiazem mat also demonstrated significant antioxidant activity by reducing tissue MDA and nitrite levels by 63% and 59% compared to normal saline. The findings support the eligibility of this novel wound dressing for additional clinical research.
Keywords: Diltiazem; electrospun nanofiber; wound healing; mouse; oxidative stress, human fibroblast
Application: Wound Dressing
Product Model 1: Electroris
Product Model 2:
URL: #https://www.tandfonline.com/doi/full/10.1080/10837450.2020.1852420#