Title: A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration
Journal: Scientific Reports
Author: 1. Hadi Samadian, 2. Saeed Farzamfar, 3. Ahmad Vaez, 4. Arian ehterami, 5. Arindam Bit, 6. Mostafa Alam, 7. Arash Goodarzi, 8. Gholamhossein Darya, 9,10. Majid Salehi
Year: 2020
Address: 1. Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medical Sciences and Technologies, Tehran University of Medical Sciences, Tehran, Iran 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Department of Mechanical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran 5. National Institute of Technology, Raipur, India 6. Department of Oral and Maxillofacial Surgery, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran 7. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran 8. Department of Comparative Biomedical Science, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran 9. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran 10. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
Abstract: The focus of the current study was to develop a functional and bioactive scafold through the combination of 3D polylactic acid (PLA)/polycaprolactone (PCL) with gelatin nanofbers (GNFs) and Taurine (Tau) for bone defect regeneration. GNFs were fabricated via electrospinning dispersed in PLA/PCL polymer solution, Tau with different concentrations was added, and the polymer solution converted into a 3D and porous scafold via the thermally-induced phase separation technique. The characterization results showed that the scafolds have interconnected pores with the porosity of up to 90%. Moreover, Tau increased the wettability and weight loss rate, while compromised the compressive strengths. The scafolds were hemo- and cytocompatible and supported cell viability and proliferation. The in vivo studies showed that the defects treated with scafolds flled with new bone. The computed tomography (CT) imaging and histopathological observation revealed that the PLA/ PCL/Gel/Tau 10% provided the highest new bone formation, angiogenesis, and woven bone among the treatment groups. Our fnding illustrated that the fabricated scafold was able to regenerate bone within the defect and can be considered as the efective scafold for bone tissue engineering application.
Keywords:
Application: Tissue Engineering, Scaffold
Product Model 1: Electroris
Product Model 2:
URL: #https://www.nature.com/articles/s41598-020-70155-2#