Title: In situ Preparation of PVA/Schizophyllan-AgNPs Nanofiber as Potential of Wound Healing: Characterization and Cytotoxicity
Journal: Fibers and Polymers
Author: 1.Hamid Kooshki, Jamal Rashidiani, 1,2. Mohammad Reza Safaee-Ardakani, 2. Ashrafalsadat Hatamian-Zarmi, Seyedeh Mahdieh Sadat, Bahman Ebrahimi-Hosseinzadeh, 3. Zahra Beagom Mokhtari-Hosseini
Year: 2019
Address: 1. Nanobiotechnology Research Center, Baqiyatallah University of Medical Science, Tehran 1435116471, Iran 2. Department of Life Sciences Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran 1439957131, Iran 3. Chemical Engineering Group, Faculty of Petroleum and Petrochemical Engineering, Hakim Sabzevari University, Sabzevar 9617976487, Iran
Abstract: The use of natural polymers and electrospinning as a new method of wound dressing production is one of the things that can revolutionize the medical world. Due to the importance of wound healing and characteristics such as antiinflammatory and antimicrobial properties, it is possible to use natural compounds such as fungi and metabolites derived from them to produce wound dressing. In this study, schizophyllan (SPG) as an extracellular polysaccharide was extracted from Iranian Schizophyllum commune (NCBI MG761830) and then the silver nanoparticles (AgNPs) were produced by the in-situ method in 1.5 % SPG solution. Afterward, they were combined with polyvinyl alcohol 10 % (PVA) polymer to strengthen the fiber structure. We investigated the properties of nanofibers containing PVA/SPG-AgNPs and PVA/SPG20 %. The physicochemical properties of two fibers were investigated by SEM, TEM, FTIR, contact angle, water uptake, nanoparticle release, and biological test (antibacterial, and MTT). The diameter of the nanofiber-containing the AgNPs was about 169 nm and the other nanofiber was about 212 nm. The highest inhibition of the growth of the bacterium by PVA/SPGAgNPs against E. coli and S. aureus was about 88.34 % and 64.7 %, respectively. The silver ion release from PVA/SPGAgNPs nanofibers was 21 µg/ml after fifth day. Both nanofibers had no toxic effect on L929 fibroblast cells.
Keywords: Schizophyllan, Nanofiber, Cytotoxicity, Silver nanoparticle, In situ
Application: Wound Dressing
Product Model 1: High Voltage Power Supply
Product Model 2:
URL: #https://link.springer.com/article/10.1007/s12221-019-9388-8#