Title: Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment
Journal: International Journal of Biological Macromolecules
Author: 1,2. Hamid Reza Bakhsheshi-Rad, 1. Mahdi Omidi, 2. Ahmad Fauzi Ismail, 2. Madzlan Aziz, 3. Mohsen Akbari, Zhina Hadisi, 4. Xiongbiao Chen
Year: 2020
Address: 1. Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran 2. Advanced Membrane Technology Research Center (AMTEC), Universiti Teknologi Malaysia, 81310, Skudai, Johor Bahru, Johor, Malaysia 3. Laboratory for Innovations in MicroEngineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, BC V8P 5C2, Canada 4. Department of Mechanical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada
Abstract: Skin and soft tissue infections are major concerns with respect to wound repair. Recently, anti-bacterial wound dressings have been emerging as promising candidates to reduce infection, thus accelerating the wound healing process. This paper presents our work to develop and characterize poly(vinyl alcohol) (PVA)/chitosan (CS)/silk sericin (SS)/tetracycline (TCN) porous nanofibers, with diameters varying from 305 to 425 nm, both in vitro and in vivo for potential applications as wound dressings. The fabricated nanofibers possess a considerable capacity to take up water through swelling (~325–650%). Sericin addition leads to increased hydrophilicity and elongation at break while decreasing fiber diameter and mechanical strength. Moreover, fibroblasts (L929) cultured on the nanofibers with low sericin content (PVA/CS/1-2SS) displayed greater proliferation compared to those on nanofibers without sericin (PVA/CS). Nanofibers loaded with high sericin and tetracycline content significantly inhibited the growth of Escherichia coli and Staphylococcus aureus. In vivo examination revealed that PVA/CS/ 2SS-TCN nanofibers enhance wound healing, re-epithelialization, and collagen deposition compared to traditional gauze and nanofibers without sericin. The results of this study demonstrate that the PVA/CS/2SS-TCN nanofiber creates a promising alternative to traditional wound dressing materials.
Keywords: Silk protein sericin, Biocompatibility, Antimicrobial performance, Wound dressing
Application: Wound Dressing
Product Model 1: Electroris
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/abs/pii/S0141813019349657#