Title: The effect of increasing the pore size of nanofibrous scaffolds on the osteogenic cell culture using a combination of sacrificial agent electrospinning and ultrasonication
Journal: Journal of Biomedical Materials Research Part
Author: 1. Mahdiyeh Aghajanpoor, Sameereh Hashemi-Najafabadi, 2. Mohamadreza Baghaban-Eslaminejad, Foruogh Azam Sayyahpour, 3. Fatemeh Bagheri, Seyyed Mohammad Mousavi
Year: 2017
Address: 1. Department of Biomedical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran 2. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran 3. Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran
Abstract: One of the major problems associated with the electrospun scaffolds is their small pore size, which limits the cellular infiltration for bone tissue engineering. In this study, the effect of increasing the pore size on cellular infiltration was studied in polycaprolactone (PCL)/ nanohydroxyapatite (nHA) electrospun scaffolds, which were modified using ultrasonication, co-electrospinning with poly (ethylene oxide) (PEO), and a combination of both. Ultrasonic process was optimized by central composite design (CCD). The ultrasonic output power and time of the process were considered as the effective parameters. The pore size of the scaffolds was evaluated by scanning electron microscope. The optimum conditions, according to the pore area and mechanical properties of the scaffolds were selected, and finally the groups that had the highest pore size and mechanical strength were selected for the combined method. Increasing the pore size enhanced the cellular proliferation, extension and infiltration, as well as the osteodifferentiation of stem cells. At the optimum condition, the average cellular infiltration was 36.51 μm compared to the control group with no cellular infiltration. In addition, alkaline phosphatase activity (ALP activity) and the expression of osteocalcin (OCN) and collagen I (COL I) were respectively 1.86, 2.54, and 2.16 fold compared to the control group on day 14.
Keywords: Bone tissue engineering, Electrospinning, Pore size, Ultrasonication, Coelectrospinning
Application: Scaffold, Tissue Engineering
Product Model 1: Electroris (ES1000)
Product Model 2:
URL: http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.36052/full#="http://onlinelibrary.wiley.com" & "/doi/10.1002/jbm.a.36052/full"#