Title: Fabrication of Collagen-Coated Poly (Beta-Hydroxybutyrate-Co-Beta-Hydroxyvalerate) Nanofiber by Chemical and Physical Methods
Journal: Oriental journal of chemistry
Author: 1. REZAEI-TAVIRANI M., 2. BIAZAR E., 3. AI J., 4. HEIDARI S., 5. ASEFNEJAD A.
Year: 2011
Address: 1,4. Clinical Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences,Tehran, (Iran). 2. Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, (Iran). 3. Department of Tissue Engineering, Faculty of Advanced Technologies, Tehran University of Medical Sciences, Tehran, (Iran). 5. Department of Biomaterial Engineering, Science and Research Branch, Islamic Azad University, Tehran, (Iran).
Abstract: Tissue engineering is the design and engineering of structures to rebuild and repair damaged tissue of the body. Poly (beta-hydroxybutyrate-co-beta-hydroxyvalerate) (PHBV) scaffold has shown good biocompatibility and biodegradable properties. Nanofibers have improved the performance of biomaterials, and could be considered effective. Electro spinning is one of the most important methods of fabricating nanofibrous scaffolding. In this study, PHBV nanofibers were well designed then modified with immobilized collagen by a chemical method (glutaraldehyde) and physical method (oxygen plasma). The samples were evaluated by attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscope (SEM), contact angle and finally, cell culture. ATR-FTIR structural analysis showed the presence of collagen on the nanofiber surfaces both the methods. The SEM images showed the average size of nanofibers to be about 280 nm, which increased with a collagen coating up to 350-400 nm in the both methods. Contact angle analysis were 67° for uncoated nanofibers and 52-56° for coated nanofibers by the both methods. Cellular investigations (unrestricted somatic stem cells) showed better adhesion and cell growth and proliferation of coated samples by physical method than chemical method.
Keywords: PHBV, Nanofiber, Collagen coating, Glutaraldehyde, Plasma treatment, Cellular investigation.
Application: Tissue Engineering
Product Model 1: Electroris
Product Model 2:
URL: http://www.orientjchem.org/dnload/REZAEITAVIRANI-M-BIAZAR-E-AI-J-HEIDARI-S-and-ASEFNEJAD-A/OJCV027I02P385-395.pdf#="http://www.orientjchem.org" & "/dnload/REZAEITAVIRANI-M-BIAZAR-E-AI-J-HEIDARI-S-and-ASEFNEJAD-A/OJCV027I02P385-395.pdf"#