Title: Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled‐releasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit
Journal: Journal of Biomedical Materials Research Part B: Applied Biomaterials
Author: 1. Majid Salehi, Mahdi Naseri-Nosar, Somayeh Ebrahimi-Barough, Jafar Ai, 2. Mohammdreza Nourani, 3. Arash Khojasteh, 4. Amir-Ali Hamidieh, 5. Amir Amani, Saeed Farzamfar
Year: 2017
Address: 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran
University of Medical Sciences, Tehran 1417755469, Iran
2. Nano Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran 1435944711, Iran
3. Dental Research Centre, Research Institute of Dental Sciences, School of Dentistry, Shaheed Beheshti University of Medical
Sciences, Tehran 1983969411, Iran
4. Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran
1411713135, Iran
5. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical
Sciences, Tehran 1417755469, Iran
Abstract: The current study aimed to enhance the efficacy of
peripheral nerve regeneration using an electrically conductive
biodegradable porous neural guidance conduit for transplantation
of allogeneic Schwann cells (SCs). The conduit was
produced from polylactic acid (PLA), multiwalled carbon
nanotubes (MWCNTs), and gelatin nanofibrils (GNFs) coated
with the recombinant human erythropoietin-loaded chitosan
nanoparticles (rhEpo-CNPs). The PLA/MWCNTs/GNFs/rhEpo-
CNPs conduit had the porosity of 85.7860.70%, the contact
angle of 77.6561.918 and the ultimate tensile strength and
compressive modulus of 5.5160.13 MPa and 2.6660.34
MPa, respectively. The conduit showed the electrical conductivity
of 0.32 S cm21 and lost about 11% of its weight after 60
days in normal saline. The produced conduit was able to
release the rhEpo for at least 2 weeks and exhibited favorable
cytocompatibility towards SCs. For functional analysis, the
conduit was seeded with 1.5 3 104 SCs and implanted into a
10 mm sciatic nerve defect of Wistar rat. After 14 weeks, the
results of sciatic functional index, hot plate latency, compound
muscle action potential amplitude, weight-loss percentage of
wet gastrocnemius muscle and Histopathological examination
using hematoxylin-eosin and Luxol fast blue staining demonstrated
that the produced conduit had comparable nerve
regeneration to the autograft, as the gold standard to bridge
the nerve gaps.
Keywords: chitosan nanoparticles, erythropoietin, neural
guidance conduit, polylactic acid, multi-walled carbon nanotubes,
Schwann cells
Application: Tissue Engineering
Product Model 1: High Voltage Power Supply
Product Model 2: Syringe Pump
URL: http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33952/full#="http://onlinelibrary.wiley.com" & "/doi/10.1002/jbm.b.33952/full"#