Title: Evaluation of wound healing efficiency of vancomycin-loaded electrospun chitosan/poly ethylene oxide nanofibers in full thickness wound model of rat
Journal: International journal of biological macromolecules
Author: 1,2. Fatemeh Kalalinia, 1,4. Nafiseh Jirofti, 1,6. Bibi Sedigheh Fazly Bazzaz, 2,7. Maryam Hashemi, Elham Pishavar, 3. Zhila Taherzadeh, 3,5. Mona Beheshti, 5. Nafise Amiri, Neda Foroghinia, 8,9. Azadeh Shahroodi, Seyed Abolghasem Sajadi Tabassi, Jebrail Movaffagh
Year: 2021
Address: 1. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran 2. Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 3. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran 4. Chemical and Biomedical Engineering Department, University of Sistan and Baluchestan, Zahedan, Iran 5. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 6. Pharmaceutical Control Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 7. Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran 8. Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran 9. Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract: Electrospun hybrid nanofibers have been extensively regarded as drug carriers. This study tries to introduce a nano fibrous wound dressing as a new strategy for a topical drug-delivery system. The vancomycin (VCM)- loaded hybrid chitosan/poly ethylene oxide (CH/PEO) nanofibers were fabricated by the blend-electrospinning process. Morphological, mechanical, chemical, and biological properties of nanofibers were examined by SEM, FTIR, release profile study, tensile assay, Alamar Blue cytotoxicity evaluation, and antibacterial activity assay. In vivo wound healing activity of hybrid CH/PEO/VCM nanofibers was evaluated in full-thickness skin wounds of rats. The hybrid CH/PEO/VCM nanofibers were successfully fabricated in a nanometer. The CH/PEO/VCM 2.5% had higher Young's Modulus, better tensile strength, smaller fiber diameterwith sustained-release profiles compared to CH/PEO/VCM5%. All nanofibers did not showany significant cytotoxicity (P<0.05)onthenormal fibroblast cells. Also, VCM-load hybrid CH/PEO nanofibers successfully inhibited bacterial growth. The wound area in the rats treated with CH/PEO/VCM 2.5% was less than CH/PEO/VCM 5% treated group. According to histological evaluation, the CH/PEO/VCM2.5% group showed the fastestwound healing than other treatment groups. Results of this study proposed that CH/PEO/VCM nanofibers could promote the wound healing process by reducing the side effects of VCM as a topical antimicrobial agent.
Keywords: Chitosan nanofibers, Vancomycin, Wound dressing
Application: Wound Dressing
Product Model 1: Electroris
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/abs/pii/S0141813021002579#