Title: In vitro and in vivo study of PCL/COLL wound dressing loaded with insulin-chitosan nanoparticles on cutaneous wound healing in rats model
Journal: International Journal of Biological Macromolecules
Author: 1. Arian Ehterami, 1,2. Majid Salehi, 3. Saeed Farzamfar, Ahmad Vaez, Arash Goodarzi, 4. Hadi Samadian, 5. Hamed Sahrapeyma, 6. Mehdi Mirzaii, 7. Sadegh Ghorbani
Year: 2018
Address: 1. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran 2. Tissue Engineering and stem cells research center, Shahroud University of Medical Sciences, Shahroud, Iran 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 4. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine Tehran University of Medical Sciences, Tehran, Iran 5. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran 6. Department of Microbiology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran 7. Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract: In the current study, insulin delivering chitosan nanoparticles were coated onto the electrospun poly (ε-caprolactone) (PCL)/Collagen (COLL) to produce a potential wound care material. Electrospun matrices were fabricated from PCL/COLL (1:1 (w/w)) solution. The insulin-loaded chitosan nanoparticles were produced by ionic gelation process and then attached onto the yarns. The dressings were investigated regarding their surface wettability, microstructure, the capacity to absorb water, water vapour permeability, mechanical properties, blood compatibility, microbial penetration, and cellular behavior. Full-thickness excisional wound model was used to assess the in vivo healing capacity of the dressings. Our data showed that after 14 days the wounds covered with PCL/COLL/Cs-Ins wound dressing could reach to nearly full wound closure compared with the sterile gauze which exhibited nearly 45% of wound size reduction. Our results suggest that fabricated scaffolds can be potentially applied in clinical practice for wound treatment.
Keywords: Poly (ε-caprolactone), Collagen, Chitosan, Insulin, Electrospinning, Skin wound-dressing
Application: Wound Dressing
Product Model 1: Syringe Pump
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/pii/S0141813018312662#